{"id":3143,"date":"2017-12-08T13:32:10","date_gmt":"2017-12-08T18:32:10","guid":{"rendered":"https:\/\/med-cgibd.sites.unc.edu\/?page_id=3143"},"modified":"2022-01-10T10:32:25","modified_gmt":"2022-01-10T15:32:25","slug":"single-cell-protein-detection-protein-simple-milo","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/","title":{"rendered":"Single Cell Protein Detection (Protein Simple Milo)"},"content":{"rendered":"<h3>Protein Simple Milo: Single Cell Western Blots<\/h3>\n<p>The Milo single-cell Western blot platform measures the expression of multiple proteins across 1,000+ cells in just 4-6 hours. Simply provide your single-cell suspension and compatible primary antibodies. The AA Core will perform the services and return fluorescence images that can be used to quantify protein expression. The Core will assist with software analysis. Milo is a powerful tool both alone and in combination with the Core\u2019s other single cell services: 10x Genomics, <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-autoprep\/\">Fluidigm C1<\/a>, and <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/biomark-hd\/\">Fluidigm BioMark HD<\/a>.<\/p>\n<p>Applications of the Milo system include:<\/p>\n<ul>\n<li>scRNA-Seq validation<\/li>\n<li>Cell subtype, protein isoform, and phospho-protein heterogeneity identification<\/li>\n<li>Histone modification detection<\/li>\n<li>Gene editing efficiency determination<\/li>\n<li>Protein expression in low-abundance samples<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3185\" src=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-5_Milo-scWest-chip.png\" alt=\"\" width=\"1200\" height=\"469\" srcset=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-5_Milo-scWest-chip.png 1200w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-5_Milo-scWest-chip-300x117.png 300w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-5_Milo-scWest-chip-768x300.png 768w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-5_Milo-scWest-chip-1024x400.png 1024w\" sizes=\"auto, (max-width: 1200px) 100vw, 1200px\" \/><\/p>\n<p>Cell capture is completed by placing 1 mL of suspended cells (10,000-100,000 cells) over a single-cell Western (scWest) chip containing thousands of tiny microwells patterned into pre-cast, photoactive polyacrylamide gel. The Milo system then lyses cells, performs rapid SDS-PAGE separation (~1 min), and immobilizes proteins using UV light. No transfer to membranes is required. Loaded chips are probed with an antibody cocktail. Chips are then ready to be imaged. Importantly, the chips can be stripped and reprobed for additional proteins.<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3186\" src=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-6_Milo-Western-details.png\" alt=\"\" width=\"1442\" height=\"303\" srcset=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-6_Milo-Western-details.png 1442w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-6_Milo-Western-details-300x63.png 300w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-6_Milo-Western-details-768x161.png 768w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/12\/Image-6_Milo-Western-details-1024x215.png 1024w\" sizes=\"auto, (max-width: 1442px) 100vw, 1442px\" \/><\/p>\n<h5><b>Instruments:\u00a0<\/b><\/h5>\n<p>Protein Simple <a href=\"https:\/\/www.proteinsimple.com\/milo.html\">Milo<\/a><\/p>\n<h5><b><br \/>\nServices:<\/b><\/h5>\n<p><strong>FACS<\/strong>: Sample preparation via FACS can be completed by the Core. Contact the <a href=\"mailto:carlton_anderson@med.unc.edu\">Associate Director<\/a> to arrange a consultation.<\/p>\n<p><strong>Cell Capture &amp; Antibody Probing<\/strong>: Please provide a single-cell suspension containing &gt;10,000 cells (10,000-100,000 cells\/mL) and up to two Western-validated primary antibodies. Primary antibodies must be generated in different species for multiplexing. The Core provides all other reagents and supplies. <a href=\"https:\/\/www.proteinsimple.com\/antibody\/milo_antibodies.html\">Click here for a selection of Milo-tested antibodies.<\/a><\/p>\n<p><strong>Imaging &amp; Analysis<\/strong>: The Core will provide scWest imaging service for the user. Fluorescence quantitation can be completed using Protein Simple\u2019s free <a href=\"http:\/\/www.proteinsimple.com\/compass\/downloads\/\">Scout Software<\/a>. The Core will assist users with software usage.<\/p>\n<h5><strong><br \/>\nPricing estimates:<\/strong><\/h5>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"37%\"><strong>Service<\/strong><\/td>\n<td width=\"20%\"><strong>Price*<\/strong><\/td>\n<td width=\"41%\"><strong>大象传媒\/NCSU\/CGIBD Member Price**<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"37%\">Cell capture, Probing, &amp; Imaging<\/td>\n<td width=\"20%\">~$730<\/td>\n<td width=\"41%\">~$470<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>* Price estimate for standard service. A customized quote can be provided upon consultation.<\/p>\n<p>** Investigators affiliated with 大象传媒, NCSU, or the CGIBD receive a ~35% discount<\/p>\n<h5><b><br \/>\nHow to start a project:<\/b><\/h5>\n<p>To begin a project using the Milo system, please contact the <a href=\"mailto:carlton_anderson@med.unc.edu\">Associate Director<\/a> to arrange a consultation.<\/p>\n<h5><b>Requirements &amp; compatibility<\/b><\/h5>\n<p><b>Sample type:<\/b> Suspension containing &gt;10,000 cells (10,000-100,000 cells\/mL)<\/p>\n<p><b>Cell diameter:<\/b> 7\u201325 \u03bcm<\/p>\n<p><b>Cell type:<\/b> Mammalian cells; in suspension and unfixed<\/p>\n<p><b>Antibody requirement:<\/b> Standard, commercial unlabeled primaries and fluorescent secondaries<\/p>\n<p><b>Other equipment needed:<\/b> Open-format fluorescence microarray scanner capable of 10 \u03bcm resolution<\/p>\n<p><b>Performance &amp; specifications<\/b><\/p>\n<p><b>Typical capture &amp; analysis yield:<\/b> 1,000\u20132,000 cells per scWest chip<\/p>\n<p><b>Molecular weight (MW) range:<\/b> 15\u2013175 kDa<\/p>\n<p><b>Typical target multiplexing:<\/b> Up to four proteins per cell based on spectral and size-based multiplexing, and antibody species compatibility. Currently, the AA core can detect up to 2 proteins per staining with the available 2-color scanner.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protein Simple Milo: Single Cell Western Blots The Milo single-cell Western blot platform measures the expression of multiple proteins across 1,000+ cells in just 4-6 hours. Simply provide your single-cell suspension and compatible primary antibodies. The AA Core will perform the services and return fluorescence images that can be used to quantify protein expression. The &hellip; <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/\" aria-label=\"Read more about Single Cell Protein Detection (Protein Simple Milo)\">Read more<\/a><\/p>\n","protected":false},"author":13588,"featured_media":4816,"parent":2193,"menu_order":15,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3143","page","type-page","status-publish","has-post-thumbnail","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Single Cell Protein Detection (Protein Simple Milo) | Center for Gastrointestinal Biology and Disease<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Single Cell Protein Detection (Protein Simple Milo) | Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"og:description\" content=\"Protein Simple Milo: Single Cell Western Blots The Milo single-cell Western blot platform measures the expression of multiple proteins across 1,000+ cells in just 4-6 hours. Simply provide your single-cell suspension and compatible primary antibodies. The AA Core will perform the services and return fluorescence images that can be used to quantify protein expression. The &hellip; Read more\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/\" \/>\n<meta property=\"og:site_name\" content=\"Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"article:modified_time\" content=\"2022-01-10T15:32:25+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1954\" \/>\n\t<meta property=\"og:image:height\" content=\"1030\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@unc_cgibd\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/\",\"url\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/\",\"name\":\"Single Cell Protein Detection (Protein Simple Milo) | Center for Gastrointestinal Biology and Disease\",\"isPartOf\":{\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png\",\"datePublished\":\"2017-12-08T18:32:10+00:00\",\"dateModified\":\"2022-01-10T15:32:25+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#primaryimage\",\"url\":\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png\",\"contentUrl\":\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png\",\"width\":1954,\"height\":1030,\"caption\":\"AA Core Banner\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.med.unc.edu\/cgibd\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"CGIBD Cores\",\"item\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Advanced Analytics Core\",\"item\":\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Single Cell Protein Detection (Protein Simple Milo)\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/#website\",\"url\":\"https:\/\/www.med.unc.edu\/cgibd\/\",\"name\":\"Center for Gastrointestinal Biology and Disease\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.med.unc.edu\/cgibd\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/#organization\",\"name\":\"Center for Gastrointestinal Biology and Disease\",\"url\":\"https:\/\/www.med.unc.edu\/cgibd\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/06\/cgibd_logo-e1558013708733.png\",\"contentUrl\":\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/06\/cgibd_logo-e1558013708733.png\",\"width\":292,\"height\":195,\"caption\":\"Center for Gastrointestinal Biology and Disease\"},\"image\":{\"@id\":\"https:\/\/www.med.unc.edu\/cgibd\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/x.com\/unc_cgibd\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Single Cell Protein Detection (Protein Simple Milo) | Center for Gastrointestinal Biology and Disease","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/","og_locale":"en_US","og_type":"article","og_title":"Single Cell Protein Detection (Protein Simple Milo) | Center for Gastrointestinal Biology and Disease","og_description":"Protein Simple Milo: Single Cell Western Blots The Milo single-cell Western blot platform measures the expression of multiple proteins across 1,000+ cells in just 4-6 hours. Simply provide your single-cell suspension and compatible primary antibodies. The AA Core will perform the services and return fluorescence images that can be used to quantify protein expression. The &hellip; Read more","og_url":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/","og_site_name":"Center for Gastrointestinal Biology and Disease","article_modified_time":"2022-01-10T15:32:25+00:00","og_image":[{"width":1954,"height":1030,"url":"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_site":"@unc_cgibd","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/","url":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/","name":"Single Cell Protein Detection (Protein Simple Milo) | Center for Gastrointestinal Biology and Disease","isPartOf":{"@id":"https:\/\/www.med.unc.edu\/cgibd\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#primaryimage"},"image":{"@id":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#primaryimage"},"thumbnailUrl":"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png","datePublished":"2017-12-08T18:32:10+00:00","dateModified":"2022-01-10T15:32:25+00:00","breadcrumb":{"@id":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#primaryimage","url":"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png","contentUrl":"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2019\/05\/AA-Core-Banner-Option-1.png","width":1954,"height":1030,"caption":"AA Core Banner"},{"@type":"BreadcrumbList","@id":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.med.unc.edu\/cgibd\/"},{"@type":"ListItem","position":2,"name":"CGIBD Cores","item":"https:\/\/www.med.unc.edu\/cgibd\/cores\/"},{"@type":"ListItem","position":3,"name":"Advanced Analytics Core","item":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/"},{"@type":"ListItem","position":4,"name":"Single Cell Protein Detection (Protein Simple Milo)"}]},{"@type":"WebSite","@id":"https:\/\/www.med.unc.edu\/cgibd\/#website","url":"https:\/\/www.med.unc.edu\/cgibd\/","name":"Center for Gastrointestinal Biology and Disease","description":"","publisher":{"@id":"https:\/\/www.med.unc.edu\/cgibd\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.med.unc.edu\/cgibd\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.med.unc.edu\/cgibd\/#organization","name":"Center for Gastrointestinal Biology and Disease","url":"https:\/\/www.med.unc.edu\/cgibd\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.med.unc.edu\/cgibd\/#\/schema\/logo\/image\/","url":"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/06\/cgibd_logo-e1558013708733.png","contentUrl":"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2017\/06\/cgibd_logo-e1558013708733.png","width":292,"height":195,"caption":"Center for Gastrointestinal Biology and Disease"},"image":{"@id":"https:\/\/www.med.unc.edu\/cgibd\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/x.com\/unc_cgibd"]}]}},"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/pages\/3143","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/users\/13588"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/comments?post=3143"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/pages\/3143\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/pages\/2193"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/media\/4816"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/cgibd\/wp-json\/wp\/v2\/media?parent=3143"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}