{"id":5572,"date":"2020-02-19T13:06:08","date_gmt":"2020-02-19T18:06:08","guid":{"rendered":"https:\/\/www.med.unc.edu\/cgibd\/?page_id=5572"},"modified":"2023-03-22T15:18:29","modified_gmt":"2023-03-22T19:18:29","slug":"conditioned-medias-for-organoid-culture","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/conditioned-medias-for-organoid-culture\/","title":{"rendered":"Conditioned Medias for Organoid Culture"},"content":{"rendered":"<p><strong>Epithelial Organoids and 2D Monolayer \u201cEpCulture&#8221; Services Offered by the Advanced Analytics Core<br \/>\n<\/strong>Organoid technologies have transformed <em>in vitro<\/em> GI tract research, but they pose unique challenges due to their non-traditional culture methods. Organoids are grown in 3D matrigel patties that require more difficult culture techniques than standard 2D methods, and their spherical structures are challenging to evaluate by microscopy. To address these challenges, investigators at 大象传媒 have pioneered 2D monolayer culture methods that use the same initial starting material as 3D organoid cultures (typically epithelium in the form of small intestinal or colonic crypts). 2D monolayers are more convenient to work with since they can be passaged and cryopreserved using standard methods. The 大象传媒 Advanced Analytics Core now offers specialized conditioned media to facilitate both 2D and 3D organoid culture. <strong>Conditioned medias are available to 大象传媒 researchers only.<\/strong><\/p>\n<h5><strong><br \/>\nConditioned Media Products and Pricing<\/strong><strong>:<\/strong><\/h5>\n<h6><strong>Note:<\/strong> For most purposes, conditioned media is supplied at 2X&#8211; this makes the effective price only $1\/mL!<\/h6>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5979\" src=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2020\/10\/CM-table.png\" alt=\"\" width=\"859\" height=\"219\" srcset=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2020\/10\/CM-table.png 859w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2020\/10\/CM-table-300x76.png 300w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2020\/10\/CM-table-768x196.png 768w, https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2020\/10\/CM-table-600x153.png 600w\" sizes=\"auto, (max-width: 859px) 100vw, 859px\" \/><\/p>\n<p>*I: Intestinal, C: Colonic, G: Gastric, P: Pancreatic, L: Liver, B: Billiary<\/p>\n<h5><\/h5>\n<h5><strong>Submitting Orders:<\/strong><\/h5>\n<p>Please email Quinn Fong (<a href=\"mailto:xequinn@med.unc.edu\">xequinn@med.unc.edu<\/a>) regarding any service requests or general inquiries<\/p>\n<h5><\/h5>\n<h5><strong>AA Core MBRB Laboratory<\/strong><\/h5>\n<p>Medical Biomolecular Research Building (MBRB)<\/p>\n<p>Room 4331<\/p>\n<p>111 Mason Farm Rd<\/p>\n<p>Chapel Hill, NC 27599<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Epithelial Organoids and 2D Monolayer \u201cEpCulture&#8221; Services Offered by the Advanced Analytics Core Organoid technologies have transformed in vitro GI tract research, but they pose unique challenges due to their non-traditional culture methods. Organoids are grown in 3D matrigel patties that require more difficult culture techniques than standard 2D methods, and their spherical structures are &hellip; <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/conditioned-medias-for-organoid-culture\/\" aria-label=\"Read more about Conditioned Medias for Organoid Culture\">Read more<\/a><\/p>\n","protected":false},"author":78759,"featured_media":0,"parent":2193,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-5572","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Conditioned Medias for Organoid Culture | Center for Gastrointestinal Biology and Disease<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/conditioned-medias-for-organoid-culture\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Conditioned Medias for Organoid Culture | Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"og:description\" content=\"Epithelial Organoids and 2D Monolayer \u201cEpCulture&#8221; Services Offered by the Advanced Analytics Core Organoid technologies have transformed in vitro GI tract research, but they pose unique challenges due to their non-traditional culture methods. Organoids are grown in 3D matrigel patties that require more difficult culture techniques than standard 2D methods, and their spherical structures are &hellip; Read more\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/conditioned-medias-for-organoid-culture\/\" \/>\n<meta property=\"og:site_name\" content=\"Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-22T19:18:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.med.unc.edu\/cgibd\/wp-content\/uploads\/sites\/466\/2020\/10\/CM-table.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@unc_cgibd\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" 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