{"id":7278,"date":"2022-01-10T10:14:58","date_gmt":"2022-01-10T15:14:58","guid":{"rendered":"https:\/\/www.med.unc.edu\/cgibd\/?page_id=7278"},"modified":"2025-04-15T10:25:10","modified_gmt":"2025-04-15T14:25:10","slug":"juno","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/juno\/","title":{"rendered":"High-Throughput Bulk Library Preparations for RNA-Seq (Fluidigm\/Standard BioTools Juno)"},"content":{"rendered":"<h5><strong>Fluidigm\/Standard BioTools Juno Library Prep, qPCR, and Genotyping Services:<\/strong><\/h5>\n<p>The 大象传媒 AA Core offers mid- to high-throughput genomics solutions using the sophisticated Juno instrument. Microfluidics technology allows sequencing libraries to be generated for up to 48 samples simultaneously with accurate, precise, and highly repeatable outcomes. Prepared libraries can be sequenced through the AA Core or returned to the user to be sequenced by their provider of choice.<\/p>\n<ul>\n<li><a href=\"https:\/\/www.fluidigm.com\/products-services\/kits-reagents-and-accessories\/genomics\/advanta-assays\">Advanta Assays<\/a>:\n<ul>\n<li><a href=\"https:\/\/www.standardbio.com\/products\/kits-reagents-and-accessories\/genomics\/advanta-assays#ngs-anchor\">Bulk RNA-Seq NGS XT Library Prep<\/a><\/li>\n<li><a href=\"https:\/\/www.standardbio.com\/products\/kits-reagents-and-accessories\/genomics\/advanta-assays#sampleid-anchor\">Sample ID Genotyping Panel<\/a><\/li>\n<li><a href=\"https:\/\/www.standardbio.com\/products\/kits-reagents-and-accessories\/genomics\/advanta-assays#solid-ngs-anchor\">Solid Tumor NGS Library Prep Assay<\/a><\/li>\n<li><a href=\"https:\/\/www.standardbio.com\/products\/kits-reagents-and-accessories\/genomics\/advanta-assays#io-anchor\">IO Gene Expression Assay<\/a><\/li>\n<li><a href=\"https:\/\/www.standardbio.com\/products\/kits-reagents-and-accessories\/genomics\/advanta-assays#rna-fusion-anchor\">RNA Fusions NGS Library Prep Assay<\/a><\/li>\n<li><a href=\"https:\/\/www.standardbio.com\/products\/kits-reagents-and-accessories\/genomics\/advanta-assays#cftr-anchor\">CFTR NGS Library Prep Assay<\/a><\/li>\n<li><a href=\"https:\/\/www.standardbio.com\/area-of-interest\/genomics-analysis\/ngs-library-prep\/targeted-sequencing\/targeted-sequencing-with-microfluidics\">Targeted DNA Sequencing Library Preparation<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h5><strong><br \/>\nInstruments: <\/strong><\/h5>\n<ul>\n<li><a href=\"https:\/\/www.standardbio.com\/support\/instrument-support\/juno-support\">Standard BioTools Juno System<\/a><\/li>\n<\/ul>\n<h5><strong>How to start a project:<\/strong><\/h5>\n<p>To begin planning a Standard BioTools Juno project with the AA Core, please contact <a href=\"mailto:ghcannon@med.unc.edu\">Gabrielle Cannon<\/a>\u00a0to arrange a consultation.<\/p>\n<h5><strong><br \/>\nFluidigm Juno Pricing Estimates:<\/strong><\/h5>\n<table style=\"border-collapse: collapse;width: 100%;height: 69px\">\n<tbody>\n<tr style=\"height: 23px\">\n<td style=\"height: 23px;width: 37%\" width=\"37%\"><strong>Service<\/strong><\/td>\n<td style=\"height: 23px;width: 19%\" width=\"19%\"><strong>Price*<\/strong><\/td>\n<td style=\"height: 23px;width: 42%\" width=\"42%\"><strong>大象传媒\/NCSU\/CGIBD Member Price**<\/strong><\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 37%;height: 23px\">Advanta Library Prep for RNA-Seq (up to 48 samples)<\/td>\n<td style=\"width: 19%;height: 23px\">$3,830<\/td>\n<td style=\"width: 42%;height: 23px\">$3,145<\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 37%;height: 23px\">Other Juno-Compatible Assays<\/td>\n<td style=\"width: 19%;height: 23px\">Available Upon Request<\/td>\n<td style=\"width: 42%;height: 23px\">Available Upon Request<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h5><\/h5>\n<h5><strong>NextSeq 500 Sequencing Pricing Estimates:<\/strong><\/h5>\n<table style=\"width: 100.312%;height: 93px\" width=\"595\">\n<tbody>\n<tr style=\"height: 23px\">\n<td style=\"width: 37.1883%;height: 23px\" width=\"303\"><strong>Service<\/strong><\/td>\n<td style=\"width: 19.9502%;height: 23px\" width=\"64\"><strong>Price*<\/strong><\/td>\n<td style=\"width: 154.72%;height: 23px\" width=\"228\"><strong>大象传媒\/NCSU\/CGIBD Member Price**<\/strong><\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 37.1883%;height: 23px\">Sequencing (1 x 75 bp, 400M reads)<\/td>\n<td style=\"width: 19.9502%;height: 23px\">$2,378<\/td>\n<td style=\"width: 154.72%;height: 23px\">$2,100<\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 37.1883%;height: 24px\">Sequencing (2 x 75 bp, 130M read pairs)<\/td>\n<td style=\"width: 19.9502%;height: 24px\">$1,953<\/td>\n<td style=\"width: 154.72%;height: 24px\">$1,675<\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 37.1883%;height: 23px\">Sequencing (2 x 75 bp, 400M read pairs)<\/td>\n<td style=\"width: 19.9502%;height: 23px\">$3,728<\/td>\n<td style=\"width: 154.72%;height: 23px\">$3,450<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>* Standard price estimate for materials and service. A customized quote can be provided upon consultation.<\/p>\n<p>** Investigators affiliated with 大象传媒, NCSU, or the CGIBD receive discounted pricing<\/p>\n<h5><\/h5>\n<h5><strong>Submitting Samples:<\/strong><\/h5>\n<p>Users may schedule an appointment to drop off samples in the AA Core genomics laboratory:<\/p>\n<h5><\/h5>\n<h5><strong>AA Core MBRB Laboratory<\/strong><\/h5>\n<p>Medical Biomolecular Research Building (MBRB)<\/p>\n<p>Room 4331<\/p>\n<p>111 Mason Farm Rd<\/p>\n<p>Chapel Hill, NC 27599<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fluidigm\/Standard BioTools Juno Library Prep, qPCR, and Genotyping Services: The 大象传媒 AA Core offers mid- to high-throughput genomics solutions using the sophisticated Juno instrument. Microfluidics technology allows sequencing libraries to be generated for up to 48 samples simultaneously with accurate, precise, and highly repeatable outcomes. Prepared libraries can be sequenced through the AA Core or &hellip; <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/juno\/\" aria-label=\"Read more about High-Throughput Bulk Library Preparations for RNA-Seq (Fluidigm\/Standard BioTools Juno)\">Read more<\/a><\/p>\n","protected":false},"author":78759,"featured_media":0,"parent":2193,"menu_order":9,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-7278","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>High-Throughput Bulk Library Preparations for RNA-Seq (Fluidigm\/Standard BioTools Juno) | Center for Gastrointestinal Biology and Disease<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/juno\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"High-Throughput Bulk Library Preparations for RNA-Seq (Fluidigm\/Standard BioTools Juno) | Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"og:description\" content=\"Fluidigm\/Standard BioTools Juno Library Prep, qPCR, and Genotyping Services: The 大象传媒 AA Core offers mid- to high-throughput genomics solutions using the sophisticated Juno instrument. 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