{"id":2239,"date":"2013-05-31T20:45:00","date_gmt":"2013-06-01T00:45:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/pep\/advancing-your-research\/"},"modified":"2024-05-27T16:38:20","modified_gmt":"2024-05-27T20:38:20","slug":"advancing-your-research","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/pep\/advancing-your-research\/","title":{"rendered":"Advancing Your Research"},"content":{"rendered":"<h2>The PEP core can advance your research<\/h2>\n<p>There are a number of ways that the PEP core can help advance research in your lab. Our most fundamental and basic ability is to produce highly pure recombinant protein. Sufficient quantities enable research in a variety of areas:<\/p>\n<ul>\n<li>Large scale enzymatic assays for screening compound libraries<\/li>\n<li>Monoclonal antibody production<\/li>\n<li>Biophysical studies like ITC and CD, the <strong><a href=\"http:\/\/www.med.unc.edu\/csb\/macinfac\" rel=\"noopener noreferrer\">Mac-N-Fac<\/a><\/strong> is great for these services<\/li>\n<li>Structural studies utilizing <a href=\"http:\/\/www.med.unc.edu\/csb\/mx\" rel=\"noopener noreferrer\"><strong>Crystallography<\/strong><\/a> or <strong><a href=\"https:\/\/www.med.unc.edu\/csb\/nmr\/\" rel=\"noopener noreferrer\">NMR<\/a>,<\/strong> both of these facilities are co-localized with this core<\/li>\n<\/ul>\n<h2>The PEP core can produce a wide variety of proteins<\/h2>\n<ul>\n<li>DNA polymerases and other utility enzymes<\/li>\n<li>Cell culture additives like growth and proliferative factors<\/li>\n<li>If you are buying it there is a chance we can make it for you<\/li>\n<\/ul>\n<h2>The PEP core can help you to generate unique reagents to advance your research program<\/h2>\n<ul>\n<li>Tagging antibodies with tags or reporters like biotin and FITC<\/li>\n<li>Production of protein with specific functional tags to enable new experiments and assays\n<ul>\n<li>Epitope tags like c-myc and HA<\/li>\n<li>Fluorescent tags like GFP<\/li>\n<li>Affinity tags like 6His and GST<\/li>\n<li>Solubility tags like MBP and CBP<\/li>\n<\/ul>\n<\/li>\n<li>Generation of stable eukaryotic cell lines producing your protein of interest to facilitate a cellular assay<\/li>\n<\/ul>\n<h2>Relevant papers of particular interest<\/h2>\n<ul>\n<li><a href=\"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full\/10.1002\/biot.201100155?casa_token=A1T4eE_R5aUAAAAA%3AKSM6ed8pyVVdSIFRZuu-eyGF9TVVr0h7u8EM_s7dDRsyumNIspKah9wjP697TapX21KgVjXgyXuxwHlhVg\">Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications<\/a><\/li>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7125906\/\">Challenges and opportunities in the purification of recombinant tagged proteins<\/a><\/li>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4741188\/\">Protein stability: a crystallographer&#8217;s perspective<\/a><\/li>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9763899\/\">Progress, applications, challenges and prospects of protein purification technology<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The PEP core can advance your research There are a number of ways that the PEP core can help advance research in your lab. Our most fundamental and basic ability is to produce highly pure recombinant protein. Sufficient quantities enable research in a variety of areas: Large scale enzymatic assays for screening compound libraries Monoclonal &hellip; <a href=\"https:\/\/www.med.unc.edu\/csb\/pep\/advancing-your-research\/\" aria-label=\"Read more about Advancing Your Research\">Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":0,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2239","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2239","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/comments?post=2239"}],"version-history":[{"count":2,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2239\/revisions"}],"predecessor-version":[{"id":2447,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2239\/revisions\/2447"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/media?parent=2239"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}