Protocols
Cell Cycle:
Propidium Iodide Staining
Other methods:聽
DNAse to Prevent Cell Clumping
from ThermoFisher
BioLegend Mitospy mitochondria stains in and
RNA Extraction From Sorted Cells
O’Flanagan et al. inBioRxIV
Rainbow Calibration Particles (we encourage all analyzer users to perform their own quality control! We recommend each lab have available some Spherotech 8-peak Rainbow Calibration beads, Cat # RCP-30-5A)