Useful Links
Spectral Viewers
Antigen Density
How does a Flow Cytometer Work?
- (with a clear explanation of what compensation is)
- from CytometryMan
Panel Building Tools
- Bio-Rad Panel Builder:听
- Panel Builder with 大象传媒 configurations. .
- (OMIPs – peer-reviewed panels designed for fluorescent assays)
Multicolor Flow Cytometry
- list from Biolegend – does not include Brilliant UV or Brilliant Blue dyes. Check BD for those.
- The from FlowJo covers tips on Compensation, software updates and links to free webinars.
- (50 minutes)
- from BD
- basics including fluorophore selection and spill over consideration from BD
- on precision multi-color panel design, spill-over, compensation, receptor density, and best practices. A fairly non-invasive registration process is required to view the videos, but worth it.
- from Biorad
- Fluorophore combinations to avoid due to increased spreading error. This new version from BD includes the Brilliant Violet and Brilliant UV dyes. Note that the configuration is for the LSRFortessa X20, which is a little different from our core instruments, but the major concepts remain.
- See what the Biexponential Data Spread looks like.
- – from Sydney Flow Cytometry
- Another for Flow Cytometry
- (Nikon Microscopy)
- Molecular Probes table of Fluorescent听
- Excyte Blog on
- Excyte Blog on 听 and
- ThermoFisher guide to calculating how many cells for a given frequency and desired CV.
- Protocols on cell preparation, staining, cell cycle from
- Cell Cycle Analysis from
- 10 Tips and Tricks for the design of multi-color panels – from Biorad
- Autofluorescence: Causes and Cures
- (Ryan Duggan鈥檚 Blog)
- – white papers and protocols (clinically oriented)
- – free antibody search platform designed to help scientists find antibodies from publications by specific experimental contexts. A.I driven, decodes published figures to help scientists find antibody usage data.
Tutorials (some require a subscription or membership)
- provides e-Learning courses in flow cytometry. Its flagship course, Flow Cytometry Essentials, is a streamlined and user-friendly educational program that demystifies common concepts and serves as a prerequisite to instrument training. Login/subscription required.
- Bio-Rad Flow Cytometry Guide:
- – free to ISAC members. Non members can pay a small fee to view specific content.
- (ThermoFisher)
- (Becton Dickinson)
- (Chromocyte)
- (Life Technologies)
- (Becton Dickinson)
Microvesicles and exosomes
- Microvescicles and viruses on the (registrations required)
RNA Detection by Flow Cytometry:
- PrimeFlow Brochure November 2014
- PrimeFlow White Paper November 2014
- (Porichis et al 2014, 5:5641)
- – Firefly is a new platform for multiplexing the measurement of up to 68 microRNAs in a high throughput format. The technology can work directly from either small volumes of biofluids or very low amounts of total RNA.
Reference Publications
- (we have a subscription – let us know if you need any methods)
- (Herzenberg et al. 2006 Nature Immunology 7:681)
- (Optimized Multicolor Immunofluorescence Panel) – a special peer-reviewed Cytometry Part A publication type that reports on newly designed and optimized multicolor panels for flow cytometry, fluorescence microscopy, image cytometry, and other polychromatic fluorescence-based methods.
Vendors Websites
- 听– contains query tool that includes 大象传媒 cytometers. Has links to basics of flow and protocols.
- – Flow Cytometry Systems
Software
- (Chromocyte)
- (ModFit, WinList, GemStone)
Freeware
- and for analysis of high content multivariate data sets (Mac OS)
- (Purdue Software)
Cytometry Groups
- (ISAC)
- (current flow issues and concerns by users over the globe)
BD Researcher’s Digest