{"id":2326,"date":"2014-04-28T16:45:00","date_gmt":"2014-04-28T20:45:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/resources-2\/protocols\/"},"modified":"2019-09-12T16:57:39","modified_gmt":"2019-09-12T20:57:39","slug":"protocols","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/resources\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"
\n

Cell Cycle<\/span>:<\/strong><\/span>
\nPropidium Iodide Staining<\/a><\/strong>
\nAnalysis of Cellular DNA Content by Flow and Laser Scanning Cytometry<\/a><\/strong><\/strong><\/p>\n

Cell Cycle using Mass Cytometry<\/a><\/strong><\/strong><\/p>\n

Other methods:\u00a0<\/span><\/p>\n

Antibody Titration<\/a><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n

DNAse to Prevent Cell Clumping<\/a><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n

Cell Filtration Options<\/a><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n

Fixation<\/a><\/strong><\/strong><\/strong><\/p>\n

Live\/Dead Cell Discrimination<\/a><\/strong><\/strong><\/strong><\/p>\n

Amine reactive beads kit for Viability Dye compensation<\/a> from ThermoFisher<\/p>\n

BioLegend Mitospy mitochondria stains in orange<\/a> and green<\/a><\/span><\/p>\n

RNA Extraction From Sorted Cells<\/a><\/strong><\/strong><\/strong><\/p>\n

Cell Prep for RNA seq\u00a0<\/a> O’Flanagan et al. inBioRxIV<\/p>\n

Rainbow Calibration Particles<\/a> (we encourage all analyzer users to perform their own quality control! We recommend each lab have available some Spherotech 8-peak Rainbow Calibration beads, Cat # RCP-30-5A)<\/span><\/strong><\/strong><\/strong><\/strong><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Cell Cycle: Propidium Iodide Staining Analysis of Cellular DNA Content by Flow and Laser Scanning Cytometry Cell Cycle using Mass Cytometry Other methods:\u00a0 Antibody Titration DNAse to Prevent Cell Clumping Cell Filtration Options Fixation Live\/Dead Cell Discrimination Amine reactive beads kit for Viability Dye compensation from ThermoFisher BioLegend Mitospy mitochondria stains in orange and green … Read more<\/a><\/p>\n","protected":false},"author":79063,"featured_media":0,"parent":2263,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2326","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2326","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/users\/79063"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/comments?post=2326"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2326\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2263"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/media?parent=2326"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}