{"id":2329,"date":"2014-04-28T16:45:00","date_gmt":"2014-04-28T20:45:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/resources-2\/useful-links\/"},"modified":"2020-03-31T14:04:32","modified_gmt":"2020-03-31T18:04:32","slug":"useful-links","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/resources\/useful-links\/","title":{"rendered":"Useful Links"},"content":{"rendered":"<div><strong><span style=\"color: #000000\">Spectral Viewers<\/span><\/strong><\/div>\n<ul>\n<li><a class=\"external-link\" title=\"BD Spectrum View\" href=\"http:\/\/www.bdbiosciences.com\/us\/s\/spectrumviewer\" target=\"_self\" rel=\"noopener noreferrer\">BD Spectrum Viewer<\/a><\/li>\n<li><a href=\"https:\/\/www.bio-rad-antibodies.com\/spectraviewer.html\">Bio-Rad Spectra Viewer<\/a><\/li>\n<li><a href=\"http:\/\/www.biolegend.com\/spectraanalyzer\" target=\"_blank\" rel=\"noopener noreferrer\">BioLegend Spectra Analyzer<\/a><\/li>\n<li><a href=\"http:\/\/www.chroma.com\/products\/fluorochrome\" target=\"_blank\" rel=\"noopener noreferrer\">Chroma<\/a><\/li>\n<li><a href=\"https:\/\/app.fluorofinder.com\/ffsv\/spectra_viewers\/d061a8a0e6e9013716f40b4ce77c43fc#\">FluoroFinder Spectra Viewer<\/a><\/li>\n<li><a style=\"color: #2a6496\" href=\"https:\/\/www.omegafilters.com\/search?search_api_fulltext=spectral+viewer\" target=\"_blank\" rel=\"noopener noreferrer\">Omega Optical Spectral Overlap Guide<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.lifetechnologies.com\/us\/en\/home\/life-science\/cell-analysis\/labeling-chemistry\/fluorescence-spectraviewer.html\" target=\"_blank\" rel=\"noopener noreferrer\">ThermoFisher Fluorescence Spectrum Viewer<\/a><\/li>\n<\/ul>\n<div><b style=\"line-height: 1.43em\">Antigen Density<\/b><\/div>\n<div>\n<ul>\n<li><a class=\"external-link\" title=\"\" href=\"\/flowcytometry\/resources-2\/expression-of-common-surface-molecules-on-blood-cells\" target=\"_self\" rel=\"noopener noreferrer\">Expression of Common Surface Molecules on Blood Cells<\/a><\/li>\n<li><a class=\"external-link\" style=\"line-height: 1.43em\" title=\"\" href=\"\/flowcytometry\/resources-2\/antigen-density-of-b-cell-markers\" target=\"_self\" rel=\"noopener noreferrer\">Antigen Density of B-Cell Markers<\/a><\/li>\n<\/ul>\n<\/div>\n<div><b style=\"line-height: 1.43em\">How does a Flow Cytometer Work?<\/b><\/div>\n<div>\n<ul>\n<li><a class=\"external-link\" style=\"line-height: 1.43em\" title=\"\" href=\"https:\/\/www.youtube.com\/watch?v=EQXPJ7eeesQ\" target=\"_self\" rel=\"noopener noreferrer\">Flow Cytometry Animation<\/a><\/li>\n<li><a class=\"external-link\" style=\"line-height: 1.43em\" title=\"\" href=\"https:\/\/www.youtube.com\/watch?v=TDRhCWaYRsg\" target=\"_self\" rel=\"noopener noreferrer\">Flow Cytometry Basics<\/a> (with a clear explanation of what compensation is)<\/li>\n<li><a href=\"https:\/\/youtu.be\/NtfPo7BJp6o\">Light Scatter Tutorial<\/a> from CytometryMan<\/li>\n<\/ul>\n<\/div>\n<div><b style=\"line-height: 1.43em\">Panel Building Tools<\/b><\/div>\n<div>\n<ul>\n<li>Bio-Rad Panel Builder:\u00a0 <a href=\"https:\/\/www.bio-rad-antibodies.com\/flow-panel-builder.html\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/www.bio-rad-antibodies.com\/flow-panel-builder.html<\/a><\/li>\n<li><a class=\"external-link\" style=\"line-height: 1.43em\" title=\"\" href=\"http:\/\/www.bdbiosciences.com\/us\/s\/gps\/\" target=\"_self\" rel=\"noopener noreferrer\">BD Horizon Guided Panel Solution (GPS)<\/a><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/unc-ch.fluorofinder.com\" target=\"_self\" rel=\"noopener noreferrer\">FluoroFinder<\/a> Panel Builder with 大象传媒 configurations. <a class=\"external-link\" title=\"\" href=\"https:\/\/fluorofinder.com\/website\/scientists\" target=\"_self\" rel=\"noopener noreferrer\">Video Tutorial<\/a>.<\/li>\n<li><a class=\"external-link\" style=\"line-height: 1.43em\" title=\"\" href=\"https:\/\/fluorofinder.com\/omips\/\" target=\"_self\" rel=\"noopener noreferrer\">Optimized Multicolor Immunofluorescence Panels via FluoroFinder<\/a> (OMIPs &#8211; peer-reviewed panels designed for fluorescent assays)<\/li>\n<\/ul>\n<\/div>\n<div><b style=\"line-height: 1.43em\">Multicolor Flow Cytometry<\/b><\/div>\n<ul>\n<li><a class=\"external-link\" title=\"\" href=\"https:\/\/www.biolegend.com\/brightness_index\" target=\"_self\" rel=\"noopener noreferrer\">Fluorophore Brightness<\/a> list from Biolegend &#8211; does not include Brilliant UV or Brilliant Blue dyes. Check BD for those.<\/li>\n<li>The <a class=\"external-link\" title=\"\" href=\"http:\/\/flowjo.typepad.com\/\" target=\"_self\" rel=\"noopener noreferrer\">Daily Dongle blog <\/a>from FlowJo covers tips on Compensation, software updates and links to free webinars.<\/li>\n<li><a class=\"external-link\" style=\"line-height: 1.43em\" title=\"\" href=\"https:\/\/youtu.be\/q0fbV5Jq9hE\" target=\"_self\" rel=\"noopener noreferrer\">Fundamentals of Flow Cytometry<\/a> (50 minutes)<\/li>\n<li><a class=\"external-link\" title=\"\" href=\"https:\/\/www.bdbiosciences.com\/documents\/Compensation_Multicolor_TechBulletin.pdf\" target=\"_self\" rel=\"noopener noreferrer\">Compensation Basics<\/a> from BD<\/li>\n<li><a class=\"external-link\" title=\"\" href=\"https:\/\/www.bdbiosciences.com\/documents\/webinar_071713_multicolor-bv.pdf\" target=\"_self\" rel=\"noopener noreferrer\">Multicolor panel design<\/a> basics including fluorophore selection and spill over consideration from BD<\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.bdbiosciences.com\/us\/applications\/research\/multicolor-flow\/m\/745795\/horizon\" target=\"_self\" rel=\"noopener noreferrer\">BD Horizon Tour videos<\/a> on precision multi-color panel design, spill-over, compensation, receptor density, and best practices. A fairly non-invasive registration process is required to view the videos, but worth it.<\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.bioradiations.com\/flow-cytometry-basic-definitions\/?WT.mc_id=sm-CORP-WW-NotProdSpec-vli_BIORADIATIONS_FLOW_CYTOMETRY_12_29_2015_mpaTCq\" target=\"_self\" rel=\"noopener noreferrer\">Basic Flow Cytometry Definitions<\/a> from Biorad<\/li>\n<li>Fluorophore combinations to avoid due to increased spreading error. <a class=\"internal-link\" title=\"\" href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2018\/06\/SpreadResolutionImpactMatrix.pdf\" target=\"_self\" rel=\"noopener noreferrer\">This new version from BD<\/a> <span style=\"color: #000000\">includes the Brilliant Violet and Brilliant UV dyes. Note that the configuration is for the LSRFortessa X20, which is a little different from our core instruments, but the major concepts remain. <\/span><\/li>\n<li><span style=\"color: #000000\">See what the <a class=\"internal-link\" title=\"\" href=\"\/flowcytometry\/resources-2\/biexponential-data-spread\" target=\"_self\" rel=\"noopener noreferrer\">Biexponential Data Spread<\/a> looks like.<\/span><\/li>\n<li><span style=\"color: #000000\"><a class=\"external-link\" title=\"PMT Voltages from Sydney Cytometry\" href=\"http:\/\/sydneycytometry.org.au\/seminars-and-tutorials\/\" target=\"_self\" rel=\"noopener noreferrer\">The impact of adjusting PMT voltages on spillover and compensation<\/a> &#8211; from Sydney Flow Cytometry <\/span><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.ebioscience.com\/resources\/application\/flow-cytometry\/antibody-fixation-considerations.htm\" target=\"_self\" rel=\"noopener noreferrer\">Antibody Fixation considerations from eBio<\/a><\/li>\n<li><a class=\"external-link\" title=\"Life Technologies School of Fluorescence\" href=\"http:\/\/www.lifetechnologies.com\/us\/en\/home\/life-science\/cell-analysis\/cell-analysis-learning-center\/molecular-probes-school-of-fluorescence\/fundamentals-of-fluorescence-microscopy\/physical-properties-that-define-fluorescence.html\" target=\"_blank\" rel=\"noopener noreferrer\">Life Technologies School of Fluorescence<\/a><\/li>\n<li><a href=\"http:\/\/nic.ucsf.edu\/dokuwiki\/doku.php?id=fluorescent_proteins\" target=\"_blank\" rel=\"noopener noreferrer\">Fuorescent Proteins Table<\/a><\/li>\n<li>Another <a class=\"external-link\" title=\"\" href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/references\/newsletters-and-journals\/bioprobes-journal-of-cell-biology-applications\/bioprobes-71\/bioprobes-71-fluorescent-proteins-attune-cytometer.html\" target=\"_self\" rel=\"noopener noreferrer\">Fluorescent Protein Table <\/a>for Flow Cytometry<\/li>\n<li><a href=\"http:\/\/www.microscopyu.com\/articles\/livecellimaging\/fpintro.html\" target=\"_blank\" rel=\"noopener noreferrer\">Introduction to Fluorescent Proteins<\/a> <span style=\"color: #000000\">(Nikon Microscopy)<\/span><\/li>\n<li>Molecular Probes table of Fluorescent\u00a0<a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/references\/molecular-probes-the-handbook\/tables\/summary-of-fluorescent-ca2-indicators-available-from-molecular-probes.html\">Calcium indicators<\/a><\/li>\n<li><a href=\"http:\/\/www.bdbiosciences.com\/documents\/Multicolor_Fluorochrome_Guide.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">BD Multicolor Fluorochrome Guide<\/a><\/li>\n<li><a href=\"http:\/\/expertcytometry.com\/category\/statistics-and-data-analysis\" target=\"_blank\" rel=\"noopener noreferrer\">Excyte Blog on Statistics and Data Analysis<\/a><\/li>\n<li>Excyte Blog on <a class=\"external-link\" title=\"\" href=\"https:\/\/www.linkedin.com\/pulse\/3-dead-cell-reagents-improve-your-data-analysis-tim-bushnell\" target=\"_self\" rel=\"noopener noreferrer\">Viability Dyes<\/a><\/li>\n<li>Excyte Blog on <a href=\"https:\/\/expertcytometry.com\/how-to-do-phospho-flow-cytometry\/\">Phospho-Flow staining<\/a>\u00a0 and <a href=\"https:\/\/expertcytometry.com\/considerations-for-assessing-protein-phosphorylation-using-flow-cytometry\/\">4 considerations for Assessing Phosphorylation using flow<\/a><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/references\/newsletters-and-journals\/bioprobes-journal-of-cell-biology-applications\/bioprobes-71\/bioprobes-71-flow-cytometry-rare-event-detection.html\" target=\"_self\" rel=\"noopener noreferrer\">How many cells do I need to collect?<\/a> ThermoFisher guide to calculating how many cells for a given frequency and desired CV.<\/li>\n<li>Protocols on cell preparation, staining, cell cycle from <a class=\"external-link\" title=\"\" href=\"https:\/\/www.bio-rad-antibodies.com\/flow-cytometry-protocols-procedures.html?utm_source=chromocyte&amp;utm_medium=email&amp;utm_campaign=flow-cytometry-webinar\" target=\"_self\" rel=\"noopener noreferrer\">Biorad<\/a><\/li>\n<li>Cell Cycle Analysis from <a href=\"https:\/\/www.miltenyibiotec.com\/US-en\/lp\/cell-cycle-analysis-using-multiparameter-flow-cytometry.html?utm_source=email&amp;utm_medium=global_singletopic&amp;utm_campaign=drugdiscovery&amp;utm_content=ca_2019wk50&amp;utm_term=flow-cytometry\">Miltenyi Biotec<\/a><\/li>\n<li>10 Tips and Tricks for the design of multi-color panels &#8211; <a class=\"external-link\" title=\"\" href=\"https:\/\/www.bio-rad-antibodies.com\/10-tips-tricks-for-the-design-of-multi-color-flow-panels.html#1\" target=\"_self\" rel=\"noopener noreferrer\">Checklist <\/a>from Biorad<\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2018\/06\/Autofluorescence.pdf\">Autofluorescence: Causes and Cures<\/a><\/li>\n<li><a href=\"http:\/\/ucflow.blogspot.de\/\" target=\"_blank\" rel=\"noopener noreferrer\">University of Chicago Flow Cytometry Lab<\/a> <span style=\"color: #000000\">(Ryan Duggan\u2019s Blog)<\/span><\/li>\n<li><span style=\"color: #000000\"><a class=\"external-link\" title=\"FlowMetric\" href=\"http:\/\/www.flowcytometryservices.com\/flow-cytometry-white-papers\" target=\"_self\" rel=\"noopener noreferrer\">FlowMetric <\/a>&#8211; white papers and protocols (clinically oriented) <\/span><\/li>\n<li><span style=\"color: #000000\"><a class=\"external-link\" title=\"\" href=\"https:\/\/www.benchsci.com\/\" target=\"_self\" rel=\"noopener noreferrer\">BenchSci <\/a>&#8211; free antibody search platform designed to help scientists find antibodies from publications by specific experimental contexts. A.I driven, decodes published figures to help scientists find antibody usage data.<\/span><\/li>\n<\/ul>\n<div><b style=\"line-height: 1.43em;color: #000000\">Tutorials<\/b> (some require a subscription or membership)<\/div>\n<ul>\n<li><a href=\"http:\/\/work-flow.tech\">Work-Flow.tech<\/a> provides e-Learning courses in flow cytometry. Its flagship course, Flow Cytometry Essentials, is a streamlined and user-friendly educational program that demystifies common concepts and serves as a prerequisite to instrument training. Login\/subscription required.<\/li>\n<li>Bio-Rad Flow Cytometry Guide: <a href=\"https:\/\/www.bio-rad-antibodies.com\/introduction-to-flow-cytometry.html\">Bio-rad\u00a0<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/flowbook.denovosoftware.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Basic Introduction to Flow Cytometry<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/cytou.org\/store\/provider\/provider09.php\" target=\"_blank\" rel=\"noopener noreferrer\">CYTO University<\/a> &#8211; free to ISAC members. Non members can pay a small fee to view specific content.<\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cell-analysis\/cell-analysis-learning-center\/molecular-probes-school-of-fluorescence\/flow-cytometry-basics.html?cid=social_20170627_73082487&amp;adbid=6285566519707602944&amp;adbpl=li&amp;adbpr=3081\" target=\"_self\" rel=\"noopener noreferrer\">Basics of Flow Cytometry<\/a> (ThermoFisher)<\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.bdbiosciences.com\/services\/training\/elearning\/index.jsp\" target=\"_blank\" rel=\"noopener noreferrer\">Flow Cytometry Web-Based training<\/a> <span style=\"line-height: 1.43em;color: #000000\">(Becton Dickinson)<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.chromocyte.com\/educate\/Beginners-Guide-Training-Resources\/Tips-for-Good-Flow-Cytometry-Experiments\" target=\"_blank\" rel=\"noopener noreferrer\">Tips for Good Flow Cytometry Experiments<\/a> <span style=\"line-height: 1.43em;color: #000000\">(Chromocyte)<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.lifetechnologies.com\/us\/en\/home\/support\/tutorials.html\" target=\"_blank\" rel=\"noopener noreferrer\">Fluorescence Tutorials<\/a> <span style=\"line-height: 1.43em;color: #000000\">(Life Technologies)<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.bdbiosciences.com\/hotlines\/webinars\/archives\" target=\"_blank\" rel=\"noopener noreferrer\">Webinar on Apoptosis, Cell Signaling, Stem Cells, T-Cell Immunology, Bead-Based Immunoassays<\/a> <span style=\"line-height: 1.43em;color: #000000\">(Becton Dickinson)<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"https:\/\/www.flowjo.com\/learn\/flowjo-university\/flowjo\" target=\"_blank\" rel=\"noopener noreferrer\">FlowJo Training (Tutorials)<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.flowcourse.co.uk\/\" target=\"_blank\" rel=\"noopener noreferrer\">Michael Ormond&#8217;s online course<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.cyto.purdue.edu\/class\/index.htm\" target=\"_blank\" rel=\"noopener noreferrer\">Paul Robinson&#8217;s Purdue Class<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.cyto.purdue.edu\/flowcyt\/educate.htm\" target=\"_blank\" rel=\"noopener noreferrer\">Other lectures on the Purdue Cyto site<\/a><\/li>\n<\/ul>\n<div><strong>Microvesicles and exosomes<\/strong><\/div>\n<div>\n<ul>\n<li><a class=\"external-link\" style=\"line-height: 1.43em\" title=\"\" href=\"https:\/\/www.miltenyibiotec.com\/US-en\/products\/macsmolecular\/reagents\/organelle-research\/exosome-isolation.html\" target=\"_self\" rel=\"noopener noreferrer\">Exosomes by Miltenyi<\/a><\/li>\n<li>Microvescicles and viruses on the <a href=\"https:\/\/offers.the-scientist.com\/webinar-high-sensitivity-flow-cytometry-enables-extracellular-vesicle-immunophenotyping-luminex-video?hsCtaTracking=4599935f-d22e-42eb-bf62-9bc69b530913%7C09832d14-b5c7-483f-a01d-1c0eb85223e4\">ImageStreamX\u00a0<\/a> (registrations required)<\/li>\n<\/ul>\n<\/div>\n<div><b style=\"line-height: 1.43em\"><b style=\"line-height: 1.43em\">RNA Detection by Flow Cytometry:<\/b><\/b><\/div>\n<div>\n<ul>\n<li><a class=\"internal-link\" title=\"\" href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2018\/06\/PrimeFlowBrochureFinal.compressed.pdf\" target=\"_self\" rel=\"noopener noreferrer\">PrimeFlow Brochure<\/a> <span style=\"color: #000000\">November 2014<\/span><\/li>\n<li><a class=\"internal-link\" title=\"\" href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2018\/06\/PrimeFlowWhitePaperFinal.compressed.pdf\" target=\"_self\" rel=\"noopener noreferrer\">PrimeFlow White Paper <\/a> <span style=\"color: #000000\">November 2014<\/span><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.nature.com\/ncomms\/2014\/141204\/ncomms6641\/full\/ncomms6641.html\" target=\"_self\" rel=\"noopener noreferrer\">Nature Communications Paper<\/a> <span style=\"color: #000000\">(Porichis et al 2014, 5:5641)<\/span><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.abcam.com\/news\/new-multiplex-assay-technology-for-rna-and-protein-quantitation\" target=\"_self\" rel=\"noopener noreferrer\">FireFly \u2013 Abcam <\/a>&#8211; <span style=\"line-height: 1.43em\">Firefly is a new platform for multiplexing the measurement of up to 68 microRNAs in a high throughput format. The technology can work directly from either small volumes of biofluids or very low amounts of total RNA.<\/span><\/li>\n<\/ul>\n<\/div>\n<div><b style=\"line-height: 1.43em\">Reference Publications<\/b><\/div>\n<ul>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/onlinelibrary.wiley.com\/book\/10.1002\/0471142956\" target=\"_blank\" rel=\"noopener noreferrer\">Current Protocols in Cytometry<\/a> <span style=\"line-height: 1.43em;color: #000000\">(we have a subscription &#8211; let us know if you need any methods)<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.nature.com\/ni\/journal\/v7\/n7\/full\/ni0706-681.html\" target=\"_blank\" rel=\"noopener noreferrer\">Interpreting Flow Cytometry Data: A Guide for the Perplexed<\/a> <span style=\"line-height: 1.43em;color: #000000\">(Herzenberg et al. 2006 Nature Immunology 7:681)<\/span><\/li>\n<li><span style=\"line-height: 1.43em;color: #000000\"><a style=\"line-height: 1.43em\" href=\"http:\/\/onlinelibrary.wiley.com\/journal\/10.1002\/%28ISSN%291552-4930\/homepage\/information_on_omips.htm\" target=\"_blank\" rel=\"noopener noreferrer\">OMPIS<\/a> (Optimized Multicolor Immunofluorescence Panel) &#8211; a special peer-reviewed <i>Cytometry Part A<\/i> publication type that reports on newly designed and optimized multicolor panels for flow cytometry, fluorescence microscopy, image cytometry, and other polychromatic fluorescence-based methods.<\/span><\/li>\n<\/ul>\n<div><strong style=\"color: #000000;line-height: 1.43em\">Vendors Websites<\/strong><\/div>\n<ul>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.amnis.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Amnis<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.bdbiosciences.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">BD Biosciences<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.beckmancoulter.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Beckman Coulter<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.biolegend.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">BioLegend<\/a><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"https:\/\/www.bio-rad-antibodies.com\/10-tips-tricks-for-the-design-of-multi-color-flow-panels.html#1\" target=\"_self\" rel=\"noopener noreferrer\">Biorad<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.cytekdev.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Cytek Development<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.ebioscience.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">eBiosciences<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.intellicyt.com\" target=\"_blank\" rel=\"noopener noreferrer\">Intellicyt<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.invitrogen.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Invitrogen<\/a><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.miltenyibiotec.com\/en\/products-and-services\/lp\/2017\/lp-us-flow-enewsletter-27306.aspx?u=abf92cce42b963bc2200dced22291013\" target=\"_self\" rel=\"noopener noreferrer\">Miltenyi<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.novusbio.com\/novusknowsflow.html\" target=\"_blank\" rel=\"noopener noreferrer\">Novus Biologicals<\/a>\u00a0<span style=\"color: #000000\">&#8211; contains query tool that includes 大象传媒 cytometers. Has links to basics of flow and protocols.<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.phnxflow.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Phoenix Flow<\/a><\/li>\n<li><a class=\"external-link\" title=\"R&amp;D Systems\" href=\"http:\/\/www.rndsystems.com\" target=\"_self\" rel=\"noopener noreferrer\">R&amp;D Systems<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.spherotech.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Spherotech<\/a><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"https:\/\/www.stemcell.com\/\" target=\"_self\" rel=\"noopener noreferrer\">StemCell Technologies<\/a><\/li>\n<li><a href=\"http:\/\/Stratedigm.com\">Stratedigm<\/a> &#8211; Flow Cytometry Systems<\/li>\n<\/ul>\n<div><b style=\"color: #000000;line-height: 1.43em\">Software<\/b><\/div>\n<ul>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.chromocyte.com\/educate\/Flow-Cytometry-Instruments-Software--Upgrades\/Flow-Cytometry-Software\" target=\"_blank\" rel=\"noopener noreferrer\">Software List<\/a> <span style=\"line-height: 1.43em;color: #000000\">(Chromocyte)<\/span><\/li>\n<li><a class=\"external-link\" title=\"\" href=\"http:\/\/www.flowjo.com\" target=\"_self\" rel=\"noopener noreferrer\">FlowJo<\/a><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.vsh.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Verity Software House<\/a> <span style=\"line-height: 1.43em;color: #000000\">(ModFit, WinList, GemStone)<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.denovosoftware.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">DeNovo Software\/FCS Express<\/a><\/li>\n<\/ul>\n<div><b style=\"color: #000000;line-height: 1.43em\">Freeware<\/b><\/div>\n<div>\n<ul>\n<li><a href=\"http:\/\/exon.niaid.nih.gov\/spice\">SPICE<\/a><span style=\"line-height: 1.43em\"> <span style=\"color: #000000\">and <\/span><\/span><a href=\"http:\/\/www.drmr.com\/pestle.zip\">PESTLE<\/a><span style=\"line-height: 1.43em\"> <span style=\"color: #000000\">for analysis of high content multivariate data sets (Mac OS)<\/span><\/span><\/li>\n<li><a href=\"http:\/\/leukobyte.com\/cytopaint-classic\">Cytopaint<\/a><\/li>\n<li><a href=\"http:\/\/www.cyto.purdue.edu\/flowcyt\/software\/Winmdi.htm\">WinMDI<\/a><\/li>\n<li><a href=\"http:\/\/www.cyto.purdue.edu\/Purdue_software\" target=\"_blank\" rel=\"noopener noreferrer\">Cytospec<\/a> <span style=\"color: #000000\">(Purdue Software)<\/span><\/li>\n<li><a href=\"http:\/\/www.cyflogic.com\/index.php\" target=\"_blank\" rel=\"noopener noreferrer\">Cyflogic<\/a><\/li>\n<li><a href=\"http:\/\/www.flowingsoftware.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Flowing<\/a><\/li>\n<\/ul>\n<\/div>\n<div><b style=\"color: #000000;line-height: 1.43em\">Cytometry Groups<\/b><\/div>\n<ul>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.isac-net.org\/\" target=\"_blank\" rel=\"noopener noreferrer\">International Society for Analytical Cytology<\/a><span style=\"line-height: 1.43em\"> (<\/span><span style=\"line-height: 1.43em;color: #000000\">ISAC<\/span><span style=\"line-height: 1.43em\">)<\/span><\/li>\n<li><a style=\"line-height: 1.43em\" href=\"http:\/\/www.cyto.purdue.edu\/hmarchiv\/index.htm\" target=\"_blank\" rel=\"noopener noreferrer\">Purdue Flow Cytometry<\/a> <span style=\"line-height: 1.43em;color: #000000\">(current flow issues and concerns by users over the globe)<\/span><\/li>\n<\/ul>\n<p><b style=\"color: #000000;line-height: 1.43em\">BD Researcher&#8217;s Digest<\/b><\/p>\n<ul>\n<li><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2020\/03\/Researchers-Digest_Vol.-1.pdf\">Volume 1<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2020\/03\/Researchers-Digest_Vol.-2.pdf\">Volume 2<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2020\/03\/BD-Researchers-Digest-March.pdf\">March 2020<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Spectral Viewers BD Spectrum Viewer Bio-Rad Spectra Viewer BioLegend Spectra Analyzer Chroma FluoroFinder Spectra Viewer Omega Optical Spectral Overlap Guide ThermoFisher Fluorescence Spectrum Viewer Antigen Density Expression of Common Surface Molecules on Blood Cells Antigen Density of B-Cell Markers How does a Flow Cytometer Work? Flow Cytometry Animation Flow Cytometry Basics (with a clear explanation &hellip; <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/resources\/useful-links\/\" aria-label=\"Read more about Useful Links\">Read more<\/a><\/p>\n","protected":false},"author":79063,"featured_media":0,"parent":2263,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2329","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/users\/79063"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/comments?post=2329"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2329\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2263"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/media?parent=2329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}