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High-Throughput Sequencing Facility (HTSF)
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Sample Processing (QAQC)
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Requesting a Quote
Requesting a Quote
Requesting a Quote
Step
1
of
3
33%
Name
*
Email
*
PI Name
*
PI Email
*
Department
*
Institution
*
Number of Samples
*
Species
*
HTSF Account Name (if Known)
Submitting Material Type
*
Raw Material
Libraries
Pools
Source Material Type
*
RNA
Total RNA
Low Concentration Total RNA
DNA
Whole Genomic DNA
ChIP DNA
Amplicons
Library Type
*
KAPA Stranded mRNA
KAPA Hyper DNA
TruSeq Stranded RNA - Ribo-Zero Gold
SMARTr/ Nextera low input RNA library
Small RNA/ miRNA libraries
16s Metagenomic
Pooling Plan
*
No Pooling
Pool in Groups
One Pool Including All Samples
If pooling in groups, how many in each group?
Sequencing Platform
*
Miseq Nano
Miseq
NextSeq 2000 P1
NextSeq 2000 P2
NextSeq 2000 P3
NovaSeq X 1.5B*
NovaSeq X 10B*
NovaSeq X 25B*
TBD(If you are unsure of the depth of sequencing needed please)
Full flowcell and partial flowcell options available. If a partial flowcell is requested, this may delay the sequencing run.
Read Type Format
*
Single End
Paired End
Preferred Cycle Length
*
Number of lanes each pool should be run on
*
Typically each pool is run on 1 lane. If unsure, put 9999
If you are unsure of the best platform for your needs, please list ether of below as preferred: We can Reply with the best platform for your need
# reads/ sample
# cluster/ sample
Goal of Sequencing/ Hypothesis
*
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