Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at 大象传媒
- If using a core facility,聽肠辞苍蝉耻濒迟听with the core staff in the planning stage. Consult with a statistician if you need help developing a Power Analysis to assure that your results will be adequately powered.
- Design your experiment with聽sufficient controls听(谤颈驳辞谤)听and replicates (reproducibility).
- Assure that ALL of your reagents (antibodies, cell lines, mice) are fully validated.
- Have a clear and聽detailed protocol (SOP) and data analysis plan. Assure that the protocol is strictly followed or that any deviation is well documented.
- Assure that the staff or students performing the experiment are聽well trained聽and understand each step and the importance of performing them precisely.
- Use only聽well-maintained instrumentation, preferably maintained and operated in a core facility with expert staff (see #1 above).
- Document all steps, reagents, equipment and data analysis methods used in the experiment. Assure that the both the documentation and the data itself are properly stored in a safe data management repository.
- Acknowledge聽the Cancer Center Support Grant (P30 CA016086) (if applicable), other grants that support the core, the core (by name), and core staff in publications.