Zeiss LSM 980
Inverted confocal microscope with听multiplexed Airyscan 2 detection for super-resolution imaging, a motorized X,Y stage and Z focus with high speed Piezo insert, and AI Sample Finder for fast sample detection and navigation.听
Location: 5114 MEJ
Modalities
- Z-stack Acquisition
- Mosaic Imaging
- AI Sample Finder
- Definite Focus
- Time-lapse Live Cell Multi-position Imaging
Acknowledgements
We suggest using the following language if the Zeiss LSM 980 was used in acquiring your images and data:
“The Zeiss LSM 980 microscope was funded with support from NIH grant S10 OD032388.”
Specifications
Objectives
| Magnification | NA | WD (mm) | Correction | Immersion | Notes |
|---|---|---|---|---|---|
| 0.16 | 18.5 | Plan-Neofluar | Air | ||
| 0.45 | 2 | Plan-Apochromat | Air | ||
| 0.8 | 0.55 | Plan-Apochromat | Air | ||
| 1.3 | 0.2 | Plan-Apochromat | Oil | ||
| 1.4 | 0.19 | Plan-Apochromat | Oil | ||
| 0.8 | 0.57 | Plan-Apochromat | Multi-immersion | Available upon request |
Lasers
| Excitation (nm) | Dyes | Type |
|---|---|---|
| 405 | Diode | |
| 488 | Diode | |
| 561 | Diode | |
| 633 | Diode |
Detectors
| Type |
|---|
| 2 flanking multialkali PMT detectors |
| 4 channel GaAsP detectors |
| Airyscan2 GaAsP array detector |
| Transmitted light PMT detector |
Software
| Version |
|---|
| ZEN Blue 3.9 |
Filter Spectra
| Filter Set | Excitation | Beamsplitter | Emission |
|---|---|---|---|
| BP 385/30 BP 469/38 BP 555/30 BP 631/33 | QBS 405 + 493 + 575 + 653 | QBP 425/30 + 514/30 + 592/25 + 709/100 | |
| BP 385/30 BP 469/38 BP 555/30 BP 631/33 | PBP 405 + 493 + 575 + 674 + 761 | PBP 425/30 + 514/31 + 592/25 + 681/45 + 785/38 |